Overview
Save time by delivering pre-made mRNAs
Save time when you want instant expression after transfection and use one of SBI’s pre-made, ready-to-transfect mRNAs. Like all of our pre-made mRNAs, the mRNAExpress™ hSox2 Transcript contains a number of different features to enhance performance:
- An anti-reverse cap analog (ARCA) to increase translation efficiency
- Modified nucleotides (5-Methylcytidine-5′-Triphosphate and Pseudouridine-5′-Triphosphate) and a poly-A tail to enhance stability and reduce host cell immune response
- Removal of the 5′ triphosphates at the end of the RNA to further reduce innate immune responses in mammalian cells
Generate induced pluripotent stem cells (iPSCs) with mRNAExpress transcripts:
| Cat.# | mRNAExpress Transcript |
|---|---|
| MR100A-1 | mRNAExpress hOct4 transcript |
| MR200A-1 | mRNAExpress hSox2 transcript |
| MR300A-1 | mRNAExpress hKlf4 transcript |
| MR400A-1 | mRNAExpress hcMyc transcript |
| MR500A-1 | mRNAExpress hLin28 transcript |
| MR600A-1 | mRNAExpress hNanog transcript |
| MR700A-1 | mRNAExpress GFP transcript (positive control) |
Supporting Data
mRNAExpress Transcripts deliver robust protein expression
Figure 1. mRNAExpress Transcripts deliver robust protein expression. HEK293 cells were transfected with 2 µg of the indicated mRNAExpress Transcript using SBI’s RNAFection™ mRNA Transfection Reagent (Cat.# MR750A-1). Autofluorescence (GFP) and immunostaining of the factors are shown in green and blue is DAPI staining for nuclei. The mRNAExpress transcripts are high quality RNAs that show the expected banding pattern with the appropriate sizes.
Resources
System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒的所有相关产品、质粒、试剂盒及相关配套试剂和慢病毒延伸产品如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。
SBI focuses on developing unique, innovative technologies that provide researchers with the tools to investigate and understand genomic and proteomic function. Our mission is to provide tools for the genome-wide analysis of the mechanisms that regulate cellular processes and biological responses.
Headquartered in Palo Alto about 30 miles south of San Francisco, SBI is geographically surrounded by highly successful research biotech companies (e.g., Affymetrix, Life Technologies, and Genentech) and some of the premier life science institutes in the world, including Stanford and the University of California, San Francisco.
As a small private biotechnology company, SBI capitalizes on a rich network of consultants and colleagues and continually collaborates to accelerate its development of innovative and novel applications. Currently, SBI has partnerships with over thirty scientists from several institutes.System Biosciences (SBI) consists of a highly motivated team committed to realizing the potential of its mission to develop and bring to market unique and innovative technology to investigate and understand genomic and proteomic function.
Possessing a diverse range of experience and knowledge, SBI's management and staff bring the skills, talent, and interest needed to support the company's continuing rapid growth.
A strong and proven background in creative research and product development with proficiency in genetic analysis, microarray technology, and cell biology
Extensive business development and management experience
A keen understanding of the needs of life scientists
Expertise in developing, marketing, and supporting consistent, high-quality research products
A clear focus on the need to build and maintain lasting customer relationships through support, service, and close customer interaction
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