Robust, controlled transgene expression with a non-integrating lentiviral system

Combining all the advantages of lentiviral vectors with the benefits of episomal expression, SBI’s pPACK-ID Integrase-defective Lentiviral Packaging Mix is a great option for researchers who want to express transgenes in mammalian cells.

Based on SBI’s popular pPACKH1 lentiviral packaging system, pPACK-ID contains targeted mutations in the POL gene which codes for the integrase protein¹. The result is a powerful lentiviral packaging system that is completely compatible with the full range of SBI’s lentiviral vectors, but without integration of the vector into the host genome. This enables transient gene expression in dividing cells and stable expression in quiescent or non-dividing cells. In addition, for researchers interested in gene therapy studies, this system supports transgene expression via lentiviral vectors without the potential risks of insertional mutagenesis.

• Safer—avoids the risks of insertional mutagenesis
• Controlled—transient expression in dividing cells, stable expression in quiescent cells
• Flexible—infect a wide range of cells with the VSV-G pseudotyped envelope
• Optimized—well-characterized virus production based on SBI’s proven pPACK system
• Ready-to-use—fully compatible with all third-generation lentiviral vectors and downstream virus concentration methods (PEG-it, ultracentrifugation, and ultrafiltration)

• The pPACK-ID-GAG plasmid contains the structural (Gag), and replication (Pol) genes which code for some of the proteins required to produce the lentivirus. It also encodes the viral Env gene, which encodes the envelope protein that defines the tropism (e. the range of infectable cells). The defective integrase is also located on this plasmid.
• The pPACK-ID-REV plasmid contains the regulatory protein Rev that is required for HIV replication.
• The pVSV-G plasmid expresses the envelope glycoprotein of vesicular stomatitis virus (VSV-G) from the CMV promoter. VSV-G pseudotyped viral particles mediate viral entry through lipid binding and plasma membrane fusion and can infect both mammalian and non-mammalian cells.

1. Sengupta, et al. An Optimized Protocol for Packaging Pseudotyped Integrase Defective Lentivirus. Biol Proced Online. July 11, 2016. 18:14. PMCID: PMC4939624.

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒的所有相关产品、质粒、试剂盒及相关配套试剂和慢病毒延伸产品如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。

SBI focuses on developing unique, innovative technologies that provide researchers with the tools to investigate and understand genomic and proteomic function. Our mission is to provide tools for the genome-wide analysis of the mechanisms that regulate cellular processes and biological responses.

Headquartered in Palo Alto about 30 miles south of San Francisco, SBI is geographically surrounded by highly successful research biotech companies (e.g., Affymetrix, Life Technologies, and Genentech) and some of the premier life science institutes in the world, including Stanford and the University of California, San Francisco.

As a small private biotechnology company, SBI capitalizes on a rich network of consultants and colleagues and continually collaborates to accelerate its development of innovative and novel applications. Currently, SBI has partnerships with over thirty scientists from several institutes.System Biosciences (SBI) consists of a highly motivated team committed to realizing the potential of its mission to develop and bring to market unique and innovative technology to investigate and understand genomic and proteomic function.

Possessing a diverse range of experience and knowledge, SBI's management and staff bring the skills, talent, and interest needed to support the company's continuing rapid growth.

A strong and proven background in creative research and product development with proficiency in genetic analysis, microarray technology, and cell biology

Extensive business development and management experience

A keen understanding of the needs of life scientists

Expertise in developing, marketing, and supporting consistent, high-quality research products

A clear focus on the need to build and maintain lasting customer relationships through support, service, and close customer interaction