A kit that streamlines genome editing at the powerful AAVS1 Safe Harbor Site

Get the critical reagents for knocking-in any gene or promoter-gene combination into the AAVS1 Safe Harbor Site with this Kit that bundles together an HR Donor—the AAVS1-Targeting All-purpose HR Donor Vector 2.0 AAVS1-SA-puro-MCS—a Cas9/AAVS1 gRNA delivery construct—the All-in-one Cas9 SmartNuclease & AAVS1 gRNA Plasmid—and a 5’ and 3’ primer mix for a Junction PCR Assay to verify integration into the AAVS1 site.

Our second generation, All-purpose HR Donor Vector 2.0 AAVS1-SA-puro-MCS is a promoterless construct enabling knock-in of any gene or promoter-gene combination into the AAVS1 site. Like all of our AAVS1 HR Donor Vectors, it come with AAVS1 homology arms already cloned in, simplifying your workflow. Just ligate-in the gene or promoter-gene combination of your choice and co-transfect with the included All-in-one Cas9 SmartNuclease & AAVS1 gRNA Plasmid. You can verify correct insertion with the included 5’ and 3’ Primer Mix for Junction PCR Assay.

Why choose one of our second generation AAVS1-targeting HR Donors?

The clever design of our second generation AAVS1-Targeting HR Donor Vectors limits off-target integration for highly-specific targeting of the AAVS1 site. Taking advantage of the AAVS1"s location within an intron, the puromycin marker has only a splice acceptor site and no promoter. Expression of puromycin can only occur when the construct integrates within an intron, reducing the probability of recovering off-target integrants in the presence of puromycin selection.

Why AAVS1?

Delivering consistent, robust transgene expression, the AAVS1 safe harbor site is a preferred target for gene knock-ins. Insertion at the site has been shown to be safe with no phenotypic effects reported, and the surrounding DNA appears to be kept in an open confirmation, enabling stable expression of a variety of transgenes.

• Easy, precise knock-in of any gene
• Consistent, robust transgene expression from the AAVS1 Safe Harbor Site
• Simplified construction of isogenic cell lines
• Minimal off-target integration
• Streamlined CRISPR/Cas9 library screening

Why use an HR targeting vector?

Even though gene knock-outs can result from DSBs caused by Cas9 alone, SBI recommends the use of HR targeting vectors (also called HR donor vectors) for more efficient and precise mutation. HR donors can supply elements for positive or negative selection ensuring easier identification of successful mutation events. In addition, HR donors can include up to 6-8 kb of open reading frame for gene knock-ins or tagging, and, when small mutations are included in either 5’ or 3’ homology arms, can make specific, targeted gene edits.

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒的所有相关产品、质粒、试剂盒及相关配套试剂和慢病毒延伸产品如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。

SBI focuses on developing unique, innovative technologies that provide researchers with the tools to investigate and understand genomic and proteomic function. Our mission is to provide tools for the genome-wide analysis of the mechanisms that regulate cellular processes and biological responses.

Headquartered in Palo Alto about 30 miles south of San Francisco, SBI is geographically surrounded by highly successful research biotech companies (e.g., Affymetrix, Life Technologies, and Genentech) and some of the premier life science institutes in the world, including Stanford and the University of California, San Francisco.

As a small private biotechnology company, SBI capitalizes on a rich network of consultants and colleagues and continually collaborates to accelerate its development of innovative and novel applications. Currently, SBI has partnerships with over thirty scientists from several institutes.System Biosciences (SBI) consists of a highly motivated team committed to realizing the potential of its mission to develop and bring to market unique and innovative technology to investigate and understand genomic and proteomic function.

Possessing a diverse range of experience and knowledge, SBI's management and staff bring the skills, talent, and interest needed to support the company's continuing rapid growth.

A strong and proven background in creative research and product development with proficiency in genetic analysis, microarray technology, and cell biology

Extensive business development and management experience

A keen understanding of the needs of life scientists

Expertise in developing, marketing, and supporting consistent, high-quality research products

A clear focus on the need to build and maintain lasting customer relationships through support, service, and close customer interaction