Get precise genomic integration of your expression cassette
Use the PrecisionX™ Gene Knock-out HR Targeting Vector (MCS1-EF1α-RFP-T2A-Puro-pA-MCS2) to knock-out any gene or edit the genome. Clone your homology arms into MCS1 and MCS2, and use puromycin selection and RFP-positive imaging to find integrants. After you’ve identified clones with your gene-of-interest knocked-out or edited, you can remove the selection cassette using the Cre-LoxP system (learn more about Cre-LoxP excision here).
Why use an HR targeting vector?
Even though gene knock-outs can result from DSBs caused by Cas9 alone, SBI recommends the use of HR targeting vectors (also called HR donor vectors) for more efficient and precise mutation. HR donors can supply elements for positive or negative selection ensuring easier identification of successful mutation events. In addition, HR donors can include up to 6-8 kb of open reading frame for gene knock-ins or tagging, and, when small mutations are included in either 5’ or 3’ homology arms, can make specific, targeted gene edits.
Choose the right HR Targeting Vector for your project
| Catalog # | HR Donor Vector | Features* | Application | |||
|---|---|---|---|---|---|---|
| Gene | Gene | Gene Edits | Gene Tagging | |||
| HR100PA-1 | MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4 | Basic HR Donor | ||||
| HR110PA-1 | MCS1-EF1α-RFP-T2A-Puro-pA-MCS2 | Removable RFP marker and puromycin selection | ||||
| HR120PA-1 | GFP-pA-LoxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCSPuro-pA-LoxP-MCS | Tag with GFP fusion Removable RFP marker and puromycin selection | ||||
| HR130PA-1 | T2A-GFP-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCSA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS | Co-express GFP with “tagged” gene via T2A Removable RFP marker and puromycin selection | ||||
| HR150PA-1 | GFP-T2A-Luc-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS | Tag with GFP fusion and co-express luciferase via T2A Removable RFP marker and puromycin selection | ||||
| HR180PA-1 | IRES-GFP-pA-loxP-MCS1-EF1α-RFP-T2A-Puro-pA-LoxP-MCS2 | Co-express GFP with “tagged” gene via IRES Removable RFP marker and puromycin selection | ||||
| HR210PA-1 | MCS1-LoxP-EF1α-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2 | Removable GFP marker, puromycin selection, and TK selection | ||||
| HR220PA-1 | GFP-pA-LoxP-EF1α-RFP-T2A-Hygro-pA-LoxP-MCS | Tag with GFP fusion Removable RFP ,arker and hygromycin Selection | ||||
| HR410PA-1 | MCS1-EF1α-GFP-T2A-Puro-pA-MCS2 | Removable GFP marker and puromycin selection | ||||
| HR510PA-1 | MCS1-EF1α-RFP-T2A-Hygro-pA-MCS2 | Removable RFP marker and hygromycin selection | ||||
| HR700PA-1 | MCS1-EF1α-GFP-T2A-Puro-pA-MCS2-PGK-hsvTK | Enrich for on-target integration with negative TK selection** Removable GFP marker and puromycin selection | ||||
| HR710PA-1 | MCS1-EF1α-RFP-T2A-Hygro-pA-MCS2-PGK-hsvTK | Enrich for on-target integration with negative TK selection** Removable RFP marker and hygromycin selection | ||||
| HR720PA-1 | MCS1-EF1α-Blasticidin-pA-MCS2-PGK-hsvTK | Enrich for on-target integration with negative TK selection** Removable blasticidin selection | ||||
| GE602A-1 | pAAVS1D-PGK-MCS-EF1α-copGFPpuro | First generation AAVS1-targeting HR Donor | ||||
| GE603A-1 | pAAVS1D-CMV-RFP-EF1α-copGFPpuro | First generation AAVS1-targeting HR Donor (positive control for GE602A-1) | ||||
| GE620A-1 | AAVS1-SA-puro-MCS | Second generation AAVS1-targeting HR Donor Promoterless to knock-in any gene or promoter-gene combination | ||||
| GE622A-1 | AAVS1-SA-puro-EF1α-MCS | Second generation AAVS1-targeting HR Donor Constitutive expression of your gene-of-interest | ||||
| GE624A-1 | AAVS1-SA-puro-MCS-GFP | Second generation AAVS1-targeting HR Donor Create reporter cell lines | ||||
| CAS620A-1 | AAVS1-SA-puro-EF1α-hspCas9 | Knock-in Cas9 to the AAVS1 site | ||||
| MCS1-5"PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3" PB TR-MCS2 | Use with the PiggyBac Transposon System Enables seamless gene editing with no residual footprint (i.e. completely remove vector sequences) | |||||
| *All HR Target Vectors except PBHR100A-1 contain LoxP sites. Any sequences that are integrated between the two LoxP sites can be removed through transient expression of Cre Recombinase. **The clever design of these HR Donors enables enrichment for on-target integration events. A PGK-hsvTK cassette is included outside of the homology arms. Because of this configuration, on-target integration that results from homologous recombination will not include the PGK-hsvTK cassette—only randomly-integrated off-target events will lead to integration of PGK-hsvTK and resulting TK activity. Therefore, TK selection will negatively select against off-target integrants. Click on any one of these vectors to see a diagram of how the negative selection works. | ||||||
System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒的所有相关产品、质粒、试剂盒及相关配套试剂和慢病毒延伸产品如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。
SBI focuses on developing unique, innovative technologies that provide researchers with the tools to investigate and understand genomic and proteomic function. Our mission is to provide tools for the genome-wide analysis of the mechanisms that regulate cellular processes and biological responses.
Headquartered in Palo Alto about 30 miles south of San Francisco, SBI is geographically surrounded by highly successful research biotech companies (e.g., Affymetrix, Life Technologies, and Genentech) and some of the premier life science institutes in the world, including Stanford and the University of California, San Francisco.
As a small private biotechnology company, SBI capitalizes on a rich network of consultants and colleagues and continually collaborates to accelerate its development of innovative and novel applications. Currently, SBI has partnerships with over thirty scientists from several institutes.System Biosciences (SBI) consists of a highly motivated team committed to realizing the potential of its mission to develop and bring to market unique and innovative technology to investigate and understand genomic and proteomic function.
Possessing a diverse range of experience and knowledge, SBI's management and staff bring the skills, talent, and interest needed to support the company's continuing rapid growth.
A strong and proven background in creative research and product development with proficiency in genetic analysis, microarray technology, and cell biology
Extensive business development and management experience
A keen understanding of the needs of life scientists
Expertise in developing, marketing, and supporting consistent, high-quality research products
A clear focus on the need to build and maintain lasting customer relationships through support, service, and close customer interaction
View Products
View Products
View Products
