Looking to isolate EVs from Tissue Culture Media or other biofluids? Check out ExoQuick-TC ULTRA.

ExoQuick ULTRA vs. Top Competitor vs. Ultracentrifugation

Figure 1. ExoQuick ULTRA delivers high yields of clean exosomes. (A) A coomassie blue-stained protein gel comparing the protein content of exosome preps isolated using different methods shows only a few, defined protein bands in the ExoQuick ULTRA lane compared to the other methods. (B) Western blotting of the gel shows that the ExoQuick ULTRA prep contains the highest levels of exosome-specific markers CD9, CD81, and Hsp70 and the lowest levels of the carryover proteins albumin and IgGH. In contrast, the prep from Company Q appears to be primarily albumin, and even the sample prepared using ultracentrifugation contains considerably higher levels of both albumin and IgGH. Each lane was loaded with 7 μg of total protein as measured using a fluorometric Qubit protein assay.

Figure 2. Fluorescent nanoparticle tracking analysis (fNTA) demonstrates the high EV yields delivered by ExoQuick ULTRA compared to Ultracentrifugation. Comparison of different isolation methods on EV yields by both volume of input serum (per mL, A) and amount of input serum protein (per mg as measured by fluorometric Qubit protein assay, B). Particle number was measured using fNTA, a technique which specifically detects EVs (see how to use fNTA to measure EV concentration at our ExoGlow-NTA page).

Benefits of ExoQuick ULTRA

• Even cleaner—reduces carry-over of albumins by 75% and immunoglobulins by 40% more than other methods, including ultracentrifugation and competitors’ kits
• Higher yields—isolate more EVs per normalized input volume than ultracentrifugation and competitors’ kits
• Better biomarker detection—see what you’ve been missing when you increase the sensitivity of EV biomarker detection by up to 11-fold (CD9) and 8-fold (CD81)
• Fast—requires less than 20-minutes of hands-on time, including a convenient column-based format for clean-up
• Cost-effective—save money with each reaction compared to how much you’ll spend using a competitor’s kit

4 Simple Stages - 20 Minutes Hands-On-Time

Uncompromising EV isolation delivers higher yields and cleaner preps

Drawing upon our years of exosome experience, the SBI team has pushed ExoQuick extracellular vesicle (EV) isolation technology to new peaks of performance with ExoQuick ULTRA for Serum & Plasma. While many EV isolation methods require you to choose between high yields, easy protocols, clean preps, and low costs, ExoQuick ULTRA is able to deliver on all these fronts for trade-off free EV preparation.

Important for intercellular communication in both normal physiology as well as disease states such as cancer, EV biology is rapidly growing field. However, obtaining EVs for in vivo and ex vivo studies can be challenging. Ultracentrifugation has been considered the gold standard for exosome isolation, but the method is time-consuming, requires large sample volume inputs and requires access to specialized equipment. Additionally, UC doesn’t isolate the cleanest exosome preparations it once was thought to produce. Other commercial kit methods are faster and easier than ultracentrifugation, but still include carryover protein that can cause over-estimation of EV amount and that can interfere with protein-sensitive studies such as mass spectrometry.

Fortunately, the SBI team is never content with good enough and has continued to improve our ExoQuick technology. Now, with our newest generation of ExoQuick—ExoQuick ULTRA—you no longer have to make the trade-off between yield, purity, speed, a simple protocol, and price. From as little as 250 μL of serum or plasma and our ExoQuick ULTRA kit, you can isolate high-quality EVs for a wide range of downstream applications such as such as western blotting, mass spectrometry, NGS sequencing, exosome labeling, and in vivo/ex vivo exosome delivery.

Each ExoQuick ULTRA Kit for Serum and Plasma comes with enough ExoQuick ULTRA (serum/plasma) reagent and convenient, pre-packed columns to complete 20 reactions*.

*1 reaction is defined as 250 μL of serum or plasma precipitated using ExoQuick ULTRA

Figure 3. EVs isolated using ExoQuick ULTRA display typical EV morphology. Transmission electron micrographs of EVs isolated from human serum using ExoQuick. The same sample is shown at two different magnifications. Multiple vesicles with typical EV morphology can be seen in each image.

Want more information on ExoQuick ULTRA? Contact us and a specialist will reach out to you within 24 hours, or speak to a specialist now at 650-968-2200.

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒的所有相关产品、质粒、试剂盒及相关配套试剂和慢病毒延伸产品如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。

SBI focuses on developing unique, innovative technologies that provide researchers with the tools to investigate and understand genomic and proteomic function. Our mission is to provide tools for the genome-wide analysis of the mechanisms that regulate cellular processes and biological responses.

Headquartered in Palo Alto about 30 miles south of San Francisco, SBI is geographically surrounded by highly successful research biotech companies (e.g., Affymetrix, Life Technologies, and Genentech) and some of the premier life science institutes in the world, including Stanford and the University of California, San Francisco.

As a small private biotechnology company, SBI capitalizes on a rich network of consultants and colleagues and continually collaborates to accelerate its development of innovative and novel applications. Currently, SBI has partnerships with over thirty scientists from several institutes.System Biosciences (SBI) consists of a highly motivated team committed to realizing the potential of its mission to develop and bring to market unique and innovative technology to investigate and understand genomic and proteomic function.

Possessing a diverse range of experience and knowledge, SBI's management and staff bring the skills, talent, and interest needed to support the company's continuing rapid growth.

A strong and proven background in creative research and product development with proficiency in genetic analysis, microarray technology, and cell biology

Extensive business development and management experience

A keen understanding of the needs of life scientists

Expertise in developing, marketing, and supporting consistent, high-quality research products

A clear focus on the need to build and maintain lasting customer relationships through support, service, and close customer interaction