Overview
Get robust, stable expression of your gene-of-interest in dividing and quiescent cells
Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-MSCV-MCS-EF1α-GFP Cloning and Expression Lentivector drives expression of your gene-of-interest from the MSCV promoter, which provides strong expression in hematopoietic and stem cells. This lentivector also enables selection for transductants using a GFP reporter.
With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
| Promoter | Expression Level | Application |
|---|---|---|
| CMV | High | Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.) |
| MSCV | High | Hematopoietic and stem cells |
| EF1α | Medium | Most cell types including primary cells and stem cells |
| PGK | Medium | Most cell types including primary cells and stem cells |
| UbC | Low | Most cell types including primary cells and stem cells |
Resources
Citations
- Shao, Y, et al. (2019) Overexpression of CXCR7 promotes mesenchymal stem cells to repair phosgene-induced acute lung injury in rats. Biomedicine & Pharmacotherapy.2019 Jan 1; 109:1233-1239. Link:Biomedicine & Pharmacotherapy
- Xu, L, et al. (2018) MiR-34c ameliorates neuropathic pain by targeting NLRP3 in a mouse model of chronic constriction injury. Neuroscience.2018 Dec 26;. PM ID:30593918
- Foerster, F, et al. (2018) Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells. Oncoimmunology.2018 Dec 26; 7(4):e1409929. PM ID:29632723
- Qi, S, et al. (2018) MCPIP1 mediates inflammatory responses induced by lipopolysaccharide and lipoteichoic acid in bovine mammary epithelial cells. Acta Biochim. Biophys. Sin. (Shanghai).2018 Dec 26;. PM ID:30590418
- Sekiba, K, et al. (2018) Pevonedistat, a first-in-class NEDD8-activating enzyme inhibitor, is a potent inhibitor of hepatitis B virus. Hepatology.2018 Dec 26;. PM ID:30586159
- Hino, N, et al. (2018) An amphipathic helix of vinexin α is necessary for substrate stiffness-dependent conformational change in vinculin. J. Cell. Sci..2018 Dec 21;. PM ID:30578314
- Ren, F, et al. (2018) The R132H mutation in IDH1 promotes the recruitment of NK cells through CX3CL1/CX3CR1 chemotaxis and is correlated with a better prognosis in gliomas. Immunol. Cell Biol..2018 Dec 21;. PM ID:30575118
- Xie, Y, et al. (2018) Molecular network of miR-1343 regulates the pluripotency of porcine pluripotent stem cells via repressing OTX2 expression. RNA Biol.2018 Dec 20;. PM ID:30567463
- Rostami, M, Haidari, K & Shahbazi, M. (2018) The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells. International Journal of Cell Biology.2018 Dec 19; 2018:1-14. Link:International Journal of Cell Biology
- Saeed, U, et al. (2018) Parvulin 14 and parvulin 17 bind to HBx and cccDNA and upregulate HBV replication from cccDNA to virion in a HBx-dependent manner. J. Virol..2018 Dec 19;. PM ID:30567987
- Vasjari, L, et al. (2018) Ras signals principally via Erk in G1 but cooperates with PI3K/Akt for Cyclin D induction and S-phase entry. Cell Cycle.2018 Dec 18;. PM ID:30560710
- Verhagen, HJMP, et al. (2018) IGFBP7 Induces Differentiation and Loss of Survival of Human Acute Myeloid Leukemia Stem Cells without Affecting Normal Hematopoiesis. Cell Rep.2018 Dec 11; 25(11):3021-3035.e5. PM ID:30540936
- Lee, PC, et al. (2018) Targeting PKCδ as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer. Cancer Cell.2018 Dec 10; 34(6):954-969.e4. PM ID:30537515
- Schoderboeck, L, et al. (2018) Genetic targeting and chemogenetic inhibition of new born neurons. Hum Gene Ther Methods.2018 Dec 7;. PM ID:30526082
- Gianni, M, et al. (2018) S100A3 a partner protein regulating the stability/activity of RARα and PML-RARα in cellular models of breast/lung cancer and acute myeloid leukemia. Oncogene.2018 Dec 7;. PM ID:30532072
- Shen, CY, et al. (2018) The extracellular SEMA domain attenuates intracellular apoptotic signaling of semaphorin 6A in lung cancer cells. Oncogenesis.2018 Dec 5; 7(12):95. PM ID:30518871
- Huang, Z, et al. (2018) Hsa-miRNA-29a protects against high glucose-induced damage in human umbilical vein endothelial cells. J. Cell. Biochem..2018 Dec 5;. PM ID:30520097
- Briard, B, et al. (2018) Fungal ligands released by innate immune effectors promote inflammasome activation during Aspergillus fumigatus infection. Nat Microbiol.2018 Dec 3;. PM ID:30510167
- Huang, YW, et al. (2018) A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder. Aging Dis.2018 Dec 1; 9(6):1043-1057. PM ID:30574417
- Briem, E, et al. (2018) MiR-203a is differentially expressed during branching morphogenesis and EMT in breast progenitor cells and is a repressor of peroxidasin. Mech. Dev..2018 Nov 30;. PM ID:30508578
System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒的所有相关产品、质粒、试剂盒及相关配套试剂和慢病毒延伸产品如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。
SBI focuses on developing unique, innovative technologies that provide researchers with the tools to investigate and understand genomic and proteomic function. Our mission is to provide tools for the genome-wide analysis of the mechanisms that regulate cellular processes and biological responses.
Headquartered in Palo Alto about 30 miles south of San Francisco, SBI is geographically surrounded by highly successful research biotech companies (e.g., Affymetrix, Life Technologies, and Genentech) and some of the premier life science institutes in the world, including Stanford and the University of California, San Francisco.
As a small private biotechnology company, SBI capitalizes on a rich network of consultants and colleagues and continually collaborates to accelerate its development of innovative and novel applications. Currently, SBI has partnerships with over thirty scientists from several institutes.System Biosciences (SBI) consists of a highly motivated team committed to realizing the potential of its mission to develop and bring to market unique and innovative technology to investigate and understand genomic and proteomic function.
Possessing a diverse range of experience and knowledge, SBI's management and staff bring the skills, talent, and interest needed to support the company's continuing rapid growth.
A strong and proven background in creative research and product development with proficiency in genetic analysis, microarray technology, and cell biology
Extensive business development and management experience
A keen understanding of the needs of life scientists
Expertise in developing, marketing, and supporting consistent, high-quality research products
A clear focus on the need to build and maintain lasting customer relationships through support, service, and close customer interaction
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