Overview
Gain the confidence to move forward with informative controls
While lentiviral transduction can be a powerful and effective method for delivering expression cassettes to target cells, enabling stable expression of genes, miRNAs, shRNAs, etc., some cell lines are transduced less efficiently than others. To help with lentiviral transduction optimization and troubleshooting, SBI offers a series of positive transduction controls that come as pre-packaged, ready-to-transduce lentiviral particles as well as ready-to-transfect and package lentivector plasmids. The pSIH1-H1-siLuc-copGFP Positive Transduction Control enables quantitation of HIV-based lentiviral transduction efficiency using the bright copGFP protein.
- Easily visualize and quantify transduction efficiency with copGFP
- Optimize transduction conditions for your cell line
- Test the sensitivity of target cells to HIV-based lentiviral transduction
pSIH1-H1-siLuc-copGFP expresses the full-length copGFP gene with human-optimized codons from the CMV promoter for high levels of expression in mammalian cells. The copGFP marker is a novel natural green monomeric GFP-like protein from copepods (Pontellina sp.). The copGFP protein is a non-toxic, non-aggregating protein with fast protein maturation and high stability at a wide range of pH (pH 4 – 12). copGFP does not require any additional cofactors or substrates and has very bright fluorescence that exceeds at least 1.3 times the brightness of EGFP, the widely used Aequorea victoria GFP mutant. The copGFP protein emits green fluorescence with the following characteristics:
- Maximum emission wavelength: 502 nm
- Maximum excitation wavelength: 482 nm
- Quantum yield: 0.6
- Extinction coefficient: 70,000 M-1 cm-1
How It Works
The lentivirus packaging, production, and transduction workflow
Supporting Data
SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism
Resources
System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒的所有相关产品、质粒、试剂盒及相关配套试剂和慢病毒延伸产品如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。
SBI focuses on developing unique, innovative technologies that provide researchers with the tools to investigate and understand genomic and proteomic function. Our mission is to provide tools for the genome-wide analysis of the mechanisms that regulate cellular processes and biological responses.
Headquartered in Palo Alto about 30 miles south of San Francisco, SBI is geographically surrounded by highly successful research biotech companies (e.g., Affymetrix, Life Technologies, and Genentech) and some of the premier life science institutes in the world, including Stanford and the University of California, San Francisco.
As a small private biotechnology company, SBI capitalizes on a rich network of consultants and colleagues and continually collaborates to accelerate its development of innovative and novel applications. Currently, SBI has partnerships with over thirty scientists from several institutes.System Biosciences (SBI) consists of a highly motivated team committed to realizing the potential of its mission to develop and bring to market unique and innovative technology to investigate and understand genomic and proteomic function.
Possessing a diverse range of experience and knowledge, SBI's management and staff bring the skills, talent, and interest needed to support the company's continuing rapid growth.
A strong and proven background in creative research and product development with proficiency in genetic analysis, microarray technology, and cell biology
Extensive business development and management experience
A keen understanding of the needs of life scientists
Expertise in developing, marketing, and supporting consistent, high-quality research products
A clear focus on the need to build and maintain lasting customer relationships through support, service, and close customer interaction
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